Scientist / Senior Scientist, Upstream Development

Precision Life Sciences - Seattle, WA

Scientist / Senior Scientist, Upstream Development

Seeking an experienced, highly motivated individual for the role of Scientist / Senior Scientist, Upstream Development. In this position, you will develop and implement suspension mammalian cell culture procedures for expression of novel gene therapy technology platform. Candidates should have expertise in early phase upstream development with specific experience in developing seed train cell culture platforms for monoclonal cell lines. The ideal candidate will have a strong problem-solving attitude, ability to effectively communicate results, ideas, and concepts to cross functional teams, and intellectual motivation to understand and optimize upstream processes for a high expression system.

Roles and Responsibilities:

  • The candidate will work as a member of the P&PD team to lead and perform upstream development to create platform processes for the expansion of stable cell lines from vial thaw to bioreactor inoculation to support rAAV viral vector production.
  • The main responsibility of this position is to perform of cell culture optimization, media development, other screening activities for optimum cell culture conditions to support high cell density, viability and superior rAAV product quality.
  • She/he will be also responsible for working with the P&PD team to support all aspects of process development from small scale screening activities to lab scale material generation for downstream and analytical development.
  • Participate in ongoing upstream technology development initiatives, as well as generating new ideas and proposals for future projects
  • As part of a growing development team, the candidate may be required to perform activities across the team in addition to upstream experiments (i.e., cell line, bioreactor, and/or analytical development).
  • Some weekend work may be necessary to maintain cell lines, perform critical monitoring, etc.


  • PhD in biochemistry, molecular biology, cell biology, bioengineering or related field with 3-5 years of industry experience in cell line development. Excellent non-PhD candidates will also be considered.
  • Demonstrated scientific rigor through peer-reviewed publications in top tier journals

Skills and Experience:

  • Proficiency with standard mammalian cell culture techniques involved in creating viral or biological therapeutics
  • Working knowledge of media development, cell culture optimization, and/or cell line engineering for optimum growth, yield and/or product quality
  • Demonstrated experience in upstream process development
  • Experience with high throughput tools, experimental design and analysis as pertains to upstream development
  • Strong background in aseptic techniques
  • Ability to collaborate and work effectively on cross functional teams, and rapidly adapt technologies and processes to meet project needs
  • Strong technical writing skills to author reports and appropriate regulatory filing sections
  • Critical thinking and clear verbal communication skills
  • Familiarity with GMP requirements and experience with regulatory documents (IND, BLA, etc.)
  • Familiarity and experience with current viral vector manufacturing techniques a plus


Posted On: Wednesday, February 1, 2023

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